© 2015 by Zenobia Therapeutics Inc

Protein Stability Product Collection

 

Definition of Fradditive™: (1) fragments of a ligand or macromolecule found bound to proteins in nature; (2) very small chemical fragments of known drugs or natural products. 

 

Why Fradditives™?

 

  • Ligands stabilize proteins in solution improving stability and crystallizability

  • Serendipitous contaminants, buffer or crystallization components have been critical for crystallizability 

  • Ligands have not been routinely incorporated into commercial screens

  • 20 years of fragment-based lead discovery data shows that fragments find binding sites on proteins

  • Not all fragments alter protein activity but are relevant to protein stability

  • We introduce a series of Fradditive™ screens to expand the diversity and productivity of ligands in protein purification and crystallization

Fradditive™ 1:  Fragments of natural products

Fradditive™ 2:  Biological building blocks and osmolytes

Fradditive™-pH: Sparse matrix sampling of Fradditive™ and buffers at a range of pH

Fradditive™-salt: Sparse matrix sampling of Fradditive™ and salts

Fradditive™ 1:  Fragments of natural products

 

No product

SOP

Screen

Contents

Questions? Contact us

  • Fradditive™ Screen 1 is composed of very small fragments (<150 MW) of natural products

  • These compounds are expected to bind specifically or non-specifically to your target to stabilize it in solution or prevent aggregation

  • We recommend screening at a 1:10 dilution

  • 0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling

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Fradditive™ 2:  Biological building blocks and osmolytes

 

No product

SOP

Screen

Contents

Questions? Contact us

  • Fradditive™ Screen 2 is composed of natural and unnatural amino acids, sugars, nucleotide and nucleotide analogues.

  • Amino acids and sugars are well-known to prevent aggregation in solution through non-specific binding.

  • These compounds may bind specifically or non-specifically to your target to stabilize it in solution during purification or crystallization.

  • We recommend screening at a 1:10 dilution.

  • 0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling

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Fradditive™ pH:  Sparse matrix sampling of Fradditive™ and buffers at a range of pH

 

No product

SOP

Screen

Contents

Questions? Contact us

  • Sparse matrix screen composed of 32 unique fragments and 32 unique buffer-pH combinations

  • Screen may be used to optimize protein purfication and crystallization

  • pH range that most commonly yield crystals is more highly sampled

  • Fradditive™ are a diverse sampling of the Fradditive™ collection (screens 1 and 2) including amino acids, nucleotides and fragments of natural products

  • We recommend screening at a 1:10 dilution

  • 0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling. Buffers are at varying concentrations. Please check the excel file for more details.

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Fradditive™ -salt:  Sparse matrix sampling of Fradditive™ and salts

 

No product

SOP

Screen

Contents

Questions? Contact us

  • Sparse matrix screen composed of 32 unique fragments and 24 unique salt combinations

  • Salts can be used to solubilize proteins and at higher concentrations used to yield crystals

  • Screen may be used to optimize protein purfication and crystallization

  • Salts that most commonly yield crystals were chosen for the screen

  • Fradditive™ are a diverse sampling of the Fradditive™ collection (screens 1 and 2) including amino acids, nucleotides and fragments of natural products

  • We recommend screening at a 1:10 dilution

  • 0.5ml of 96 aqueous solutions are provided in deep well blocks to facilitate high-through sampling.  Salts are at varying concentrations.  Please check excel file for details.

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Academic and quantity discounts available. 

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