Protein Stability Product Collection
Definition of Fradditive™: (1) fragments of a ligand or macromolecule found bound to proteins in nature; (2) very small chemical fragments of known drugs or natural products.
Why Fradditives™?
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Ligands stabilize proteins in solution improving stability and crystallizability
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Serendipitous contaminants, buffer or crystallization components have been critical for crystallizability
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Ligands have not been routinely incorporated into commercial screens
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20 years of fragment-based lead discovery data shows that fragments find binding sites on proteins
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Not all fragments alter protein activity but are relevant to protein stability
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We introduce a series of Fradditive™ screens to expand the diversity and productivity of ligands in protein purification and crystallization
Fradditive™ 1: Fragments of natural products
Fradditive™ 2: Biological building blocks and osmolytes
Fradditive™-pH: Sparse matrix sampling of Fradditive™ and buffers at a range of pH
Fradditive™-salt: Sparse matrix sampling of Fradditive™ and salts
Fradditive™ 1: Fragments of natural products
No product
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Fradditive™ Screen 1 is composed of very small fragments (<150 MW) of natural products
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These compounds are expected to bind specifically or non-specifically to your target to stabilize it in solution or prevent aggregation
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We recommend screening at a 1:10 dilution
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0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling
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Fradditive™ 2: Biological building blocks and osmolytes
No product
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Fradditive™ Screen 2 is composed of natural and unnatural amino acids, sugars, nucleotide and nucleotide analogues.
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Amino acids and sugars are well-known to prevent aggregation in solution through non-specific binding.
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These compounds may bind specifically or non-specifically to your target to stabilize it in solution during purification or crystallization.
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We recommend screening at a 1:10 dilution.
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0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling
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Fradditive™ pH: Sparse matrix sampling of Fradditive™ and buffers at a range of pH
No product
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Sparse matrix screen composed of 32 unique fragments and 32 unique buffer-pH combinations
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Screen may be used to optimize protein purfication and crystallization
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pH range that most commonly yield crystals is more highly sampled
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Fradditive™ are a diverse sampling of the Fradditive™ collection (screens 1 and 2) including amino acids, nucleotides and fragments of natural products
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We recommend screening at a 1:10 dilution
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0.5ml of 96 10mM aqueous solutions are provided in deep well blocks to facilitate high-through sampling. Buffers are at varying concentrations. Please check the excel file for more details.
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Fradditive™ -salt: Sparse matrix sampling of Fradditive™ and salts
No product
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Sparse matrix screen composed of 32 unique fragments and 24 unique salt combinations
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Salts can be used to solubilize proteins and at higher concentrations used to yield crystals
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Screen may be used to optimize protein purfication and crystallization
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Salts that most commonly yield crystals were chosen for the screen
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Fradditive™ are a diverse sampling of the Fradditive™ collection (screens 1 and 2) including amino acids, nucleotides and fragments of natural products
-
We recommend screening at a 1:10 dilution
-
0.5ml of 96 aqueous solutions are provided in deep well blocks to facilitate high-through sampling. Salts are at varying concentrations. Please check excel file for details.